The Rowland Institute for Science.

Movies

-Swimming E. coli

-Swimming Rhodobacter

-Swimming Synechococcus

-Swarming Salmonella

-Swarming Serratia

-Twitching Pseudomonas

-Gliding Cytophaga

-Gliding Mycoplasma

-Tethered bacteria

-E. coli patterns

-Miscellaneous movies


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Swimming Escherichia coli

Introduction

Most of these movies were made of fluorescently-labeled cells with strobed laser illumination, using an ordinary CCD camera at 60 Hz, as described by Turner et al. (2000). However, the playback speeds vary. Others were made of fluorescently labeled cells at 500 Hz, without the strobe. Movies in phase contrast used tungsten-halogen illumination and an ordinary CCD camera. These cells where grown on T-broth. They are about 1 µm in diameter by 2 µm long and swim at about 30 µm/s. For more information, see the project Fluorescent flagella.

Movies, cells in fluorescence

Fluorescent filaments on a stuck cell
Fluorescent filament leaving bundle
Fluorescent cells near the slide
Fluorescent semi-coiled bundle
Fluorescent curly 1 bundle
Fluorescent bundles
Fluorescent bundles, 500 Hz

Movies, cells in phase contrast

Cells near the slide, then above the slide

Reference

Turner, L., Ryu, W.S., and Berg, H.C. "Real-time imaging of fluorescent flagellar filaments." J. Bacteriol. 182, 2793-2801 (2000).

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Copyright © 2003 The Rowland Institute for Science.
Last modified Tuesday, July 23, 2003.