Basarab Gabriel Hosu
Department of Molecular and Cellular Biology
Harvard Bio Labs
16 Divinity Avenue, Room 3063
Cambridge, MA 02138
Phone: 617-495-4217, Fax: 617-496-1114
Rowland Institute at Harvard
100 Edwin H. Land Blvd.
Cambridge, MA 02142
Phone: 617-497-4606, Fax: 617-497-4627
I am working on an experiment designed to tell us whether cytoplasmic motor components (e.g., components of the C-ring) spin at the same rate as extracellular components (e.g., the hook and filament). A cytoplasmic component, labeled with a fluorescent protein embedded in the C-ring, is bleached by an intense flash of polarized light. The fluorophores that survive are excited by a weak beam of polarized light. As the C-rings rotate, these fluorophores will fluoresce when aligned with the plane of polarization of the excitation beam. Thus, following the bleaching flash, the fluorescence emission should ring at twice the ring rotation frequency.
Hosu, B.G., Nathan, V.S.J., and Berg, H.C. Internal and external components of the bacterial flagellar motor rotate as a unit. Proc. Natl. Acad. Sci. USA 113, 4783-4787 (2016). Featured in MCB News article ()
Lele, P. P., Hosu, B. G., Berg, H. C. Dynamics of mechanosensing in the bacterial flagellar motor, Proc. Natl. Acad. Sci. 110, (29), 11839-11844 (2013). ()
Yuan, J., Branch, R., Hosu, B.G., and Berg, H.C. Adaptation at the output of the chemotaxis signalling pathway. Nature 484, 233-236 (2012).
Wu, Y., Hosu, B.G., and Berg, H.C. Microbubbles reveal chiral fluid flows in bacterial swarms. Proc. Natl. Acad. Sci. USA 108, 4147-4151 (2011).
Hosu, B.G., Mullen, S.F., Critser, J.K. and Forgacs, G. Reversible disassembly
of the actin cytoskeleton improves the survival rate and developmental
competence of cryopreserved mouse oocytes. PLoS ONE 3(7), e2787
Hosu, B.G., Sun, M., Marga, F., Grandbois, M. and Forgacs, G. Eukaryotic
membrane tethers revisited using magnetic tweezers. Phys Biol. Apr
19; 4(2), 67-78 (2007).
Hosu, B.G., Jakab, K., Banki, P., Toth, F.I. and Forgacs, G. Magnetic tweezers
for intracellular applications. Rev Sci Instrum. 74(9), 4158-4163 (2003).
Kovacs, E., Pologea-Moraru, R. and Hosu, B.G. Evaluation of viability of
retinal photoreceptor cells by using their endogenous electrical field. Bioelectrochemistry 56(1-2), 219-21