People -Howard Berg -Karen Fahrner -Basarab Gabriel Hosu -Donna Lundberg -Veda Nathan -Linda Turner Stern -Yilin Wu -Junhua Yuan -Rongjing Zhang

Linda Turner Stern Research Interests I am interested in the motility of micro-organisms, especialy how bacteria swim (move as individuals in a liquid) and swarm (move in coordinated packs across a surface). During both behaviors the bacterial flagellar filament propels the bacterium. This filament is composed of many copies of a single protein, called flagellin, arranged in a spiralling path on a cylindrical surface. The overall shape of the filament is helical. Remarkably, this rigid helical propellor changes shape, undergoing polymorphic transformations, as the bacterium swims and swarms. I enjoy thinking about and sorting out the relationship between applied motor torque, hydrodynamics, transformation and behavior. I use fluorescent labeling, color imaging, and high speed imaging, http://www.jclabs.com/, to understand the role of the flagella in cell behavior. Selected Publications Hulme, S.E., DiLuzio, W.R., Shevkoplyas, S.S., Turner, L., Mayer, M., Berg, H.C. and Whitesides, G.W. Using ratchets and sorters to fractionate motile cells of Escherichia coli by length. Lab on a Chip xxx, 1-8 (2008) Berke, A.P., Turner, L., Berg, H.C. and Lauga, E. Hydrodynamic attraction of swimming microorganisms by surfaces. Phys. Rev. Lett. 101, 038102 (2008) Blair, K., Turner, L., Winkelman, J., Berg, H.C. and Kearns, D.B. A molecular clutch disables flagella in the Bacillus subtilis biofilm. Science 320, 1636-1638 (2008). Chen, B.G., Turner, L. and Berg, H.C. The wetting agent required for swarming in Salmonella enterica serovar Typhimurium is not a surfactant. J Bacteriol. 189, 8750-8753 (2007). Darnton, N., Turner, L., Rojevsky, S. and Berg, H.C. On torque and tumbling in swimming Escherichia coli. J. Bacteriol. 189, 1756-1764 (2007). Yi, D.K., Kim, M.J., Turner, L., Breuer, K.S. and Kim, D. Colloid lithography-induced polydimethylsiloxane microstructures and their application to cell patterning. Biotechnol. Lett. 28, 169–173 (2006). DiLuzio, W.R., Turner, L, Mayer, M., Garstecki, P., Weibel, D.B., Berg, H.C. and Whitesides, G.M. Escherichia coli swim on the right-hand side. Nature 435, 1271-4 (2005). Darnton, N., Turner, L., Breuer, K. and Berg H.C. Moving fluid with bacterial carpets. Biophys J. 86, 1863-70 (2004). Turner, L., Ryu, W.S. and Berg, H.C. Real-time imaging of fluorescent flagellar filaments. J. Bacteriol. 182, 2793-2801 (2000). Turner, L., Samuel, A.D.T., Stern, A.S. and Berg, H.C. Temperature dependence of switching of the bacterial flagellar motor by the protein CheY13DK106YW. Biophys. J. 77, 597-603 (1999). Scharf, B.E., Fahrner, K.A., Turner, L. and Berg, H.C. Control of direction of flagellar rotation in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 95, 201-206 (1998). Turner, L., Caplan, S.R. and Berg, H.C. Temperature-induced switching of the bacterial flagellar motor. Biophys. J. 71, 2227-2233 (1996). Berry, R.M., Turner, L. and Berg, H.C. Mechanical limits of bacterial flagellar motors probed by electrorotation. Biophys. J. 69, 280-286 (1995). Berg, H.C. and Turner, L. Cells of Escherichia coli swim either end forward. Proc. Natl. Acad. Sci. USA 92, 477-479 (1995). Berg, H.C. and Turner, L. Torque generated by the flagellar motor of Escherichia coli. Biophys. J. 65, 2201-2216 (1993). Berg, H.C. and Turner, L. Selection of motile nonchemotactic mutants of Escherichia coli by field-flow fractionation. Proc. Natl. Acad. Sci. USA 88, 8145-8148 (1991). Berg, H.C. and Turner, L. Chemotaxis of bacteria in glass capillary arrays. Biophys. J. 58, 919-930 (1990). Other Professional Activities Symposium Organizer, Material Research Society, Symposium AA: Molecular Motors, Nanomachines and Engineered Systems, San Francisco, CA, April 17-21, 2006 Invited speaker, Frontiers in the Interaction between Physics and Biology, Spring 2004, Brown University, Providence, RI Contributor, National Nanotechnology Initiative, Workshop on NanoBiotechnology, Oct. 2003 Arlington, VA Invited speaker, American Society for Microbiology, Conference on Bio-, Micro-, and Nanosystems, July 2003, NYC, NY

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