stacks are useful for a variety of purposes in imaging.
Recently, we have demonstrated that fluid flow can
transport a sample through focus with nanometer
accuracy. Using fluid flow, focus stacks of
individual cells can be produced in a single millisecond
at throughputs of 100's of focus stacks per second.
channel tilted at an angle transports cells through
Collected focus stacks of a red blood cell and a
application of focus stacks is to produce phase images
(using the Transport of Intensity Equation) that quantify
optical path differences across the cell. Phase
images can be used to resolve cellular features that are
not visible in bright-field images or to quantify cell
morphology in depth.
|Phase image of a red blood
cell in flow taking a "parachute" shape
image of a Leukemia cell in flow, where the
pathlength of sub-cellular features can be
S. S. Gorthi and E. Schonbrun, "Phase imaging flow cytometry using a focus-stack
collecting microscope," Opt. Lett, 37, 707-709 (2012).